Inhibition of cytoplasmic cap methylation identifies 5′ TOP mRNAs as recapping targets and reveals recapping sites downstream of native 5′ ends
نویسندگان
چکیده
منابع مشابه
RNA guanine-7 methyltransferase catalyzes the methylation of cytoplasmically recapped RNAs
Cap homeostasis is a cyclical process of decapping and recapping that impacts a portion of the mRNA transcriptome. The metastable uncapped forms of recapping targets redistribute from polysomes to non-translating mRNPs, and recapping is all that is needed for their return to the translating pool. Previous work identified a cytoplasmic capping metabolon consisting of capping enzyme (CE) and a 5'...
متن کاملRecapping Laminoplasty for Adult Low-Grade Isthmic Spondylolisthesis
Study design: Prospective study. Objectives of the study: To assess the results of recapping laminoplasty with posterior instrumentation and fusion in the management of adult low grade isthemic spondylolithesis. Background: Isthmic spondylolisthesis is a condition where there is a translational displacement of the upper vertebral body compared to the lower one secondary to a defect in the pars ...
متن کاملexploring interconnections between iranian efl teachers beleifs about the status of english as an international language (efl), use of mother tongue in efl classroom, and diffrences between native and non-native teachers of english
in one pole of the continuum of language learning, theoreticians and practitioners traditionally consider english as english as a second language or english as a foreign language. however, in the other pole of this continuum where english is thought of as the most effective tool for international communication, it is referred to as a lingua franca. those who favor an approach to english growing...
15 صفحه اولPerturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites.
N6-methyladenosine (m6A) is a common modification of mRNA with potential roles in fine-tuning the RNA life cycle. Here, we identify a dense network of proteins interacting with METTL3, a component of the methyltransferase complex, and show that three of them (WTAP, METTL14, and KIAA1429) are required for methylation. Monitoring m6A levels upon WTAP depletion allowed the definition of accurate a...
متن کاملThe messenger RNA decapping and recapping pathway in Trypanosoma.
The 5' terminus of trypanosome mRNA is protected by a hypermethylated cap 4 derived from spliced leader (SL) RNA. Trypanosoma brucei nuclear capping enzyme with cap guanylyltransferase and methyltransferase activities (TbCgm1) modifies the 5'-diphosphate RNA (ppRNA) end to generate an m7G SL RNA cap. Here we show that T. brucei cytoplasmic capping enzyme (TbCe1) is a bifunctional 5'-RNA kinase ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2020
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkaa046